Separation, Electroanalytical and Spectrochemical Techniques
Makokha, V. (Vincent) (2010)
This module consists of three interrelated subject areas; Separation Techniques and Chromatographic Techniques, Electro analytical Techniques and Spectroscopic Methods. The module will be taught in six learning units reflecting common concepts and approaches. Separation Techniques and Chromatographic Techniques unit will review elementary separation techniques that are usually taught in the school system, followed by a discussion of Chromatography Techniques these are covered by introducing the general chromatographic theory, followed by its application in different techniques of plane and column chromatographic techniques. Electro Analytical Techniques will introduce principles on which potentiometry is based, elaborate the common applications of potentiometry, this will be followed by voltammetry, starting with polarographic techniques ending with cyclic and anodic stripping voltammetry. The unit on Spectroscopy and Atomic Spectroscopic Techniques will review concept of energy matter interaction, concepts of energy levels in atoms and molecules, and the unit will end with a discussion of atomic spectroscopic techniques. Molecular Spectroscopy 1 will start with a discussion of the theory of UV-Visible spectroscopy, how it arises and how it is used in qualitative and quantitative analysis, instrumentation of the modern UV-visible spectrophotometer. The unit will end with a discussion of infrared spectroscopy starting with how the spectra arises, the different peaks exhibited by specific functional groups and how to apply IR in identification of functional groups and compounds. Molecular Spectroscopy 2 will introduce nuclear magnetic resonance phenomenon, followed by a discussion of proton NMR, the relationship of chemical shift with the molecular chemical environment and how proton NMR used in identification of functional groups. The unit ends with a discussion of the carbon NMR and how it compliments proton NMR in analysis of compounds. The last learning unit will be Mass Spectrometry starting with how mass spectra arises, how it is used in identification of organic compounds ending with the Instrumentation for mass spectrometry.
African Virtual University (AVU)